v.1. To put some amount into the pool at the beginning of a round in poker or a similar card game: Everyone should ante up $1 to start the game. It may be your last dollar, but you'll have to ante it up! We must ante up before the cards are dealt.
2. To provide some funds or capital: The fundraisers anted up $10,000 for the charity.
3. To pay some amount of money, often reluctantly: Travelers are forced to ante up $5 for a candy bar at the airport. Can you imagine having to ante money up to use the restroom?